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. 2010 Jun 21;59(9):2306–2314. doi: 10.2337/db09-1727

FIG. 2.

FIG. 2.

Characteristics of the granulation tissue in SV129 mice. Several morphological characteristics of the granulation tissue were evaluated in WT and p66Shc knockout (KO), diabetic (DM) (4-week duration), and nondiabetic (ND) mice with or without hind limb ischemia. Masson trichrome staining allowed determination of collagen area and granulation tissue thickness. Reepithelialization was quantified in histological sections by measuring the percentage of re-covered epithelial gap. Capillary density within the granulation tissue was quantified by B4 isolectin immunofluorescence. Apoptosis was assessed with the ApopTag kit in the granulation tissue and at wound edges, a critical site for active wound healing. Representative images are shown for control (nondiabetic nonischemic) and diabetic ischemic WT and KO mice. n ≥ 3 mice for each group; n ≥ 5 sections for tissue samples. *P < 0.05 in KO vs. corresponding WT group. †P < 0.05 in diabetic vs. corresponding nondiabetic group. (A high-quality digital representation of this figure is available in the online issue.)