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. Author manuscript; available in PMC: 2011 Jul 21.
Published in final edited form as: J Am Chem Soc. 2010 Jul 21;132(28):9789–9796. doi: 10.1021/ja102585v

Figure 5.

Figure 5

Multiplexed confocal analysis of hAuNP-labeled RSV mRNA with other mRNA sequences and subcellular organelles. (A) F protein was detected by monoclonal antibody in RSV-infected HEp-2 (MOI = 0.25) (B) RSV-specific hAuNP emission (C) DIC overlay. (D) Simultaneous imaging of RSV mRNA (red) and GAPDH mRNA (blue) using spectrally-distinct hAuNP indicated areas of distinct localization and colocalization within the cell. (E) RSV-targeted hAuNP were observed to colocalize with Mitotracker(MT)-stained mitochondria, but are also observed in distinct locations within RSV-infected cells.