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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Structure. 2009 Nov 11;17(11):1433–1441. doi: 10.1016/j.str.2009.09.008

Figure 2. Localization Accuracy of Immobilized Quantum Dots.

Figure 2

(A) The point-spread-function of a single QD-NGF molecule overlaid with the fitted 2D Gaussian function. The central position is determined to high accuracy after 2D Gaussian fitting.

(B) Spread of center positions of an immobilized quantum dot over 321 frames.

(C) Dependence of localization accuracy versus the brightness of the quantum dot. By excluding points below 5000 a.u., a centroid standard deviation of 11.5 nm was achieved.

(D) The distribution of the standard deviations of 30 QD-NGF molecules imaged over 800 frames (80 s), with the mean at 11.5 nm.