Fig 6.

Fast phase kinetics of iron chelation from iron citrate by DFP and DFO alone or in combination using stopped flow. Reactions between iron citrate and either DFO (A), DFP (B) or DFO plus DFP (C) are shown. Iron citrate (20:200 µM) in 20 mM MOPS pH 7.4 and DFO (trace A: 20 µM) or DFP (trace B: 60 µM) or DFP plus DFO (trace C: 20 µM DFO, 60 µM DFP) were placed into the two syringes of a stopped flow apparatus. On autotrigger, the syringes ejected an equal volume of both solutions into the reaction chamber where the reaction was monitored by measuring changes in absorbance at 460 nm. Reaction chamber concentrations are half those given for the syringes.