Fig 8.

Rate of iron complex formation from iron citrate albumin incubated with DFO or DFP measured by spectrophotometry. DFO (■, 10 µM) or DFP (▲, 30 µM) was incubated with iron citrate albumin (10:100 µM:40 g/l) in 20mM MOPS (pH 7.4) at RT and the reaction continuously monitored for 8h at 460 nm by spectrophotometer. Iron complex concentrations were calculated using previously determined extinction coefficients for both iron complexes. The data shown are the mean ± SE of 3 independent experiments. Inset: Black trace: incubation of DFP (30µM) with iron citrate albumin was repeated over a shorter time scale (15 minutes) to determine the reaction profile. Grey trace: DFO (10 µM) and DFP (30 µM) were co-incubated with iron citrate albumin for 20 min and the measured absorbance at 460 nm converted to feroxamine concentration.