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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Mol Psychiatry. 2006 Jun 27;11(11):992–1002. doi: 10.1038/sj.mp.4001863

Figure 4.

Figure 4

CRF1 receptor antagonists block stress-induced pCREB and Fos expressions in immature hippocampus. (a,b) The CRF1 antagonist NBI 30775 blocks stress-induced pCREB expression in the hippocampus. In a rat pretreated with saline, pCREB is highly expressed in CA3 (and CA1) immediately after the termination of a 30-min stress (a). This stress-induced pCREB expression is blocked (fully in CA3, partially in CA1) by infusion of NBI 30775 (b). (c) The peripheral, hormonal response to the ‘psychological’ stress is robust (P < 0.05), and is not influenced by icv administration of either saline or CRH receptor antagonists including the selective CRF1 blocker NBI 30775 or the general blocker α-helical CRH. (d–f) Infusion of CRF1 antagonists block Fos expression in CA3. (d) At 60 min after stress termination, Fos is strongly expressed in CA3 of the P18 rat that had received icv saline 30 min prior to stress onset. In contrast, stress-induced Fos expression is practically abrogated by infusion of NBI 30775 (e) or α-helical CRH (f), 30 min prior to stress onset. Scale bar = 720 μm (a–f) and 80 μm (inset in b).