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. 2010 Jun 16;10:295. doi: 10.1186/1471-2407-10-295

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Copyright ©2010 Kalinina et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunostaining and cell surface expression of epithelial/pancreatic markers and overexpressed proteins in PaCa 5061 cells. 2A. The formaldehyde fixed and agar embedded cells were immunostained for the presence of pancreatic cancer markers CA 19-9 and CEA respectively, as well as for cytokeratin as epithelial cell marker proteins (upper panel). Several on RNA-level overexpressed genes (Microarray) were chosen. To confirm protein overexpression of selected amplified genes in PaCa 5061 cells immunostaining was performed for Mucin4, EGFR and CEACAM 6 (lower panel). 2B. FACS profiles of PaCa 5061 cells. Cell surface expression of CD44, EpCAM, EGFR, CEACAM1, CEACAM5 and CEACAM6 were obtained with specific antibodies as in materials and methods. Each histogram shows cell surface expression of the corresponding marker (filled curves) and the irrelevant, isotype-matched antibody (open curves).