Figure 5.

LC3 phosphomimic shows reduced recruitment and suppresses neurite degeneration. (a and b) SH-SY5Y cells expressing GFP-LC3–WT or –S12D treated with rapamycin or vehicle for 1 h. Quantification of GFP-LC3 puncta. *, P = 2.31 × 10⁻⁸ versus vehicle/WT; **, P = 0.004 versus rapamycin/WT (n = 70–80 cells/condition). (c) Quantification of GFP-LC3 puncta in SH-SY5Y 48 h after transfection. *, P = 0.001 versus empty vector/GFP-LC3–WT; **, P = 0.025 versus LRRK2 G2019S/GFP-LC3–WT (n = 100–115 cells/condition). (d) Quantification of GFP-LC3 puncta in SH-SY5Y cells treated with MPP⁺ or vehicle for 48 h. *, P = 0.026 versus vehicle/WT; **, P = 0.043 versus MPP⁺/WT (n = 85–95 cells/condition). (e) Quantification of neurite lengths from SH-SY5Y cells 48 h after transfection. *, P = 6.37 × 10⁻⁴ versus empty vector/WT; **, P = 0.008 versus LRRK2 G2019S/WT (n = 100–115 cells/condition). (f) Quantification of neurite lengths from SH-SY5Y cells treated with MPP⁺ or vehicle for 48 h. *, P = 3.58 × 10⁻⁸ versus vehicle/WT; **, P = 3.74 × 10⁻⁴ versus MPP⁺/WT (n = 85–95 cells/condition). (g and h) Quantification of neurite length and branch points in cortical neurons coexpressing either GFP-LC3–WT or –S12A with empty vector or LRRK2 G2019S. *, P < 0.01 versus empty vector/WT; **, P < 0.036 versus LRRK2 G2019S/WT (n = 30–40 cells/condition). Error bars indicate mean ± SEM. Bars, 20 µm.