Table 2.
|
|
|
Subtype |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Wild-type codon (consensus B) NRTI mutations | Mutation | Samples from experienced patients n (%/57) | A (n = 10) | AE (n = 5) | AG (n = 1) | B (n = 28) | C (n = 5) | DF (n = 2) | F (n = 2) | G (n = 2) | H (n = 2) |
M41 | L | 8 (14.0) | — | — | — | 7 | 1 | — | — | — | — |
E44 | A | 1 (1.7) | 1 | — | — | — | — | — | — | — | — |
D | 3 (5.3) | — | — | — | 2 | 1 | — | — | — | — | |
D67 | G | 2 (3.5) | 1 | — | — | 1 | — | — | — | — | — |
N | 12 (21.0) | 1 | 2 | — | 7 | 2 | — | — | — | — | |
T69 | D | 1 (1.7) | — | — | — | 1 | — | — | — | — | — |
K70 | E | 1 (1.7) | — | — | — | 1 | — | — | — | — | — |
R | 9 (15.8) | 1 | 2 | — | 3 | 2 | 1c | — | — | — | |
L74 | V | 3 (5.3) | 1 | — | — | 2 | — | — | — | — | — |
V75 | M | 6 (10.5) | 4 | — | — | 2 | — | — | — | — | — |
V118 | I | 5 (8.8) | — | — | — | 3 | — | — | 2 | — | — |
Q151 | M | 1 (1.7) | 1 | — | — | — | — | — | — | — | — |
M184 | Vd | 24 (42.1) | 4 | 1 | 1 | 10 | 4 | 1 | 1 | 1 | 1 |
L210 | W | 7 (12.3) | 1 | — | — | 6 | — | — | — | — | — |
T215 | F | 6 (10.5) | 1 | 1 | — | 2 | 2 | — | — | — | — |
I | 3 (5.3) | — | 1 | — | 1 | 1 | — | — | — | — | |
Y | 8 (14.0) | 1 | — | — | 6 | — | — | — | 1 | — | |
S | 1 (1.7) | — | — | — | — | 1 | — | — | — | — | |
K219 | E | 3 (5.3) | — | 1 | — | 2 | — | — | — | — | — |
Q | 7 (12.3) | — | 1 | — | 3 | 2 | 1 | — | — | — | |
N | 3 (5.3) | — | — | — | 3 | — | — | — | — | — |
NNRTI mutations | n (%/14) | A (n = 4) | AE (n = 1) | AG (n = 0) | B (n = 5) | C (n = 1) | DF (n = 2) | F (n = 1) | G (n = 1) | H (n = 0) | |
---|---|---|---|---|---|---|---|---|---|---|---|
A98 | G | 1 (7.1) | — | — | — | 1 | — | — | — | — | — |
K103 | N | 2 (14.3) | — | — | — | — | — | 1 | 1 | — | — |
V106 | M | 1 (7.1) | — | 1 | — | — | — | — | — | — | — |
V179 | D | 1 (7.1) | 1 | — | — | — | — | — | — | — | — |
G190 | A | 1 (7.1) | — | 1 | — | — | — | — | — | — | — |
Q | 2 (14.3) | 1 | — | — | 1 | — | — | — | — | — | |
S | 1 (7.1) | — | — | — | — | — | 1 | — | — | — | |
P225 | H | 1 (7.1) | — | — | — | — | — | 1 | — | — | — |
PI mutationse | n (%/46) | A (n = 7) | AE (n = 4) | AG (n = 1) | B (n = 23) | C (n = 5) | DF (n = 2) | F (n = 0) | G (n = 1) | H (n = 2) | |
---|---|---|---|---|---|---|---|---|---|---|---|
L24 | I | 1 (2.2) | — | — | — | 1 | — | — | — | — | — |
D30 | N | 2 (4.3) | — | — | — | 1 | 1 | — | — | — | — |
L33 | F | 1 (2.2) | — | 1 | — | — | — | — | — | — | — |
M46 | I | 4 (8.7) | — | — | — | 3 | — | 1 | — | — | — |
L | 1 (2.2) | — | — | — | 1 | — | — | — | — | — | |
G48 | V | 2 (4.3) | — | — | — | 1 | 1 | — | — | — | — |
I50 | V | 1 (2.2) | — | — | — | — | 1 | — | — | — | — |
V82 | A | 2 (4.3) | — | 1 | — | 1 | — | — | — | — | — |
F | 2 (4.3) | — | — | — | 1 | — | 1 | — | — | — | |
T | 1 (2.2) | — | — | — | 1 | — | — | — | — | — | |
I84 | V | 6 (13.0) | — | — | — | 5 | — | 1 | — | — | — |
L90 | M | 13 (28.3) | — | — | — | 10 | 1 | 1 | — | — | 1 |
|
|
|
Subtype |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Wild-type codon (consensus B) Other PI mutationsf | Mutation | Overall n (%/46) | A (n = 7) | AE (n = 4) | AG (n = 1) | B (n = 23) | C (n = 5) | DF (n = 2) | F (n = 0) | G (n = 1) | H (n = 2) |
I13 | V | 20 (43.5) | 7 | 3 | 1 | 4 | 1 | 1 | — | 1 | 2 |
G16 | E | 7 (15.5) | 1 | 2 | — | 1 | 1 | — | — | — | 2 |
K20 | I | 2 (4.3) | — | — | 1 | 1 | — | — | — | — | — |
M | 1 (2.2) | — | — | — | 1 | — | — | — | — | — | |
R | 3 (6.5) | — | 1 | — | 1 | — | — | — | — | 1 | |
T | 2 (4.3) | — | — | — | 1 | 1 | — | — | — | — | |
L33 | V | 4 (8.7) | — | — | —4 | — | — | — | — | — | — |
M36 | I | 21 (45.6) | 6 | 4 | 1 | 3 | 4 | — | — | 1 | 2 |
L | 3 (6.5) | — | — | — | 1 | 2 | — | — | — | — | |
F53 | L | 1 (2.2) | — | — | — | 1 | — | — | — | — | — |
Y | 1 (2.2) | — | — | — | — | 1 | — | — | — | — | |
Q58 | E | 3 (6.5) | — | 1 | — | 1 | 1 | — | — | — | — |
D60 | E | 6 (13.0) | — | — | — | 2 | 3 | — | — | — | 1 |
I62 | V | 18 (39.1) | 2 | — | — | 14 | 1 | — | — | — | 1 |
L63 | P | 22 (47.8) | 1 | — | — | 16 | 3 | 1 | — | 1 | — |
I64 | L | 2 (4.3) | — | — | — | 2 | — | — | — | — | — |
M | 1 (2.2) | — | — | — | — | 1 | — | — | — | — | |
V | 4 (8.7) | — | — | — | 4 | — | — | — | — | — | |
H69 | K | 23 (50.0) | 6 | 4 | 1 | 4 | 5 | — | — | 1 | 2 |
A71 | I | 1 (2.2) | — | — | — | 1 | — | — | — | — | — |
T | 2 (4.3) | — | — | — | 2 | — | — | — | — | — | |
V | 5 (10.9) | — | — | — | 4 | — | 1 | — | — | — | |
T74 | A | 3 (6.5) | — | — | — | 1 | 2 | — | — | — | — |
P | 1 (2.2) | — | — | — | 1 | — | — | — | — | — | |
S | 4 (8.7) | 2 | — | — | 2 | — | — | — | — | — | |
V77 | I | 5 (10.9) | — | — | — | 4 | — | 1 | — | — | — |
V82 | I | 4 (8.7) | — | — | — | 2 | 1 | — | — | 1 | — |
N83 | D | 1 (2.2) | — | 1 | — | — | — | — | — | — | — |
L89 | V | 2 (4.3) | — | — | — | 1 | 1 | — | — | — | — |
I93 | L | 10 (21.7) | — | 1 | — | 5 | 3 | 1 | — | — | — |
M | 3 (6.5) | 1 | — | — | 2 | — | — | — | — | — |
The analysis was focused on the mutations associated with ARV resistance8 (HIV Drug Resistance Database: http://hivdb.stanford.edu) and was performed on 58 sequences of the protease gene and 57 of the reverse transcriptase gene.
NRTI, nucleoside reverse transcriptase inhibitor; NNRTI, non-NRTI; PI, protease inhibitor.
The amino acid R present at position 70 represents the wild-type codon in the DF subtype.
The prevalence of M184V resulted in a significant (by Fisher exact test) difference between the HIV-1 A or B subtype compared to the C subtype (21% vs. 80%, p = 0.025 and 24% vs. 80%, p = 0.028, respectively).
Mutations in the protease gene that confer high, intermediate, or low resistance to PIs.
Mutations in the protease gene that only contribute to PI resistance.