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. 2008 Sep;24(9):1175–1183. doi: 10.1089/aid.2008.0062

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Copyright 2008, Mary Ann Liebert, Inc.

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FIG. 3. — HIV-peptide-induced TRAIL-secreting cells are CD8 positive. (A) CD8 T cell depletion. Full PBMCs (solid bars) and CD8 cell-depleted PBMCs (open bars, <0.01% residual CD8⁺ T cells) were tested in a TRAIL ELISPOT assay for reactivity to the specified peptides as described in Fig. 1. Results are shown for an individual donor showing the mean and SD of spot numbers induced by the peptide tested in duplicate wells. The data are representative for 121 individual HIV peptides inducing TRAIL production in 6 donors. (B) CD8⁺ T cell enrichment. Bulk PBMCs (solid bars) and purified CD8⁺ T cells as obtained by negative selection (hatched bars, >97% purity) were tested in a TRAIL ELISPOT assay as described above. Results are depicted for an individual donor showing the mean and SD of spot numbers induced by each specified peptide tested in duplicate wells. The data are representative for 72 individual HIV peptides inducing TRAIL production in 4 donors.