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. 2008 Sep;24(9):1175–1183. doi: 10.1089/aid.2008.0062

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Copyright 2008, Mary Ann Liebert, Inc.

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FIG. 4. — Representative ELISPOT wells showing HIV-peptide-induced production of TRAIL or IFN-γ by CD8⁺ T cells. (A–D) HIV-peptide-induced ELISPOTs are shown on the right with the corresponding medium control wells on the left. (E) Spot size distribution histogram for TRAIL and IFN-γ spots produced by PBMCs in HIV⁺CD4^low donors, as specified. Cumulative data obtained by analysis of 2500 spots in each category are shown. (F) Spontaneous TRAIL release in HIV⁺CD4^low (n = 21), HIV⁺CD4^high (n = 8), and HIV⁻CD4^high (n = 22) control donors. Each data point represents the mean of duplicate wells for a single donor with SD <20%.