Table 1.
|
% of peptides to which a positive response was detecteda |
|||||||
---|---|---|---|---|---|---|---|---|
A | B | C | D | E | F | G | Mean ± SD | |
IFN-γ | 7 | 10.7 | 4.7 | 7.4 | 0 | 0 | 8.3 | 5.4 ± 3.8 |
TRAIL | 74.4 | 67.9 | 76.3 | 78.6 | 33.3 | 58.3 | 50 | 62.7 ± 15.3 |
IFN-γ/TRAIL | 18.6 | 21.4 | 19 | 14.3 | 66.7 | 41.7 | 41.7 | 31.9 ± 17.6 |
PBMCs of seven HIV+CD4low subjects (A–G) were tested in ELISPOT assays for HIV-peptide-induced production of IFN-γ or TRAIL; 78 HIV peptides were tested individually in duplicate wells. In each individual the number of peptides inducing a positive response (out of the 78 peptides tested) was set as 100%. We then calculated the percentage of peptides that induced IFN-γ alone (in the absence of TRAIL), TRAIL alone (in the absence of IFN-γ), or the combination of both. Mean and SD for all subjects have been calculated and are shown in the last column.