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Merged phase-contrast (hepatocytes) and red-fluorescent LSECs. Images taken 2 h post-LSEC seeding. (A) Hepatocytes–LSECs and (B) Hepatocytes-5 PE Layers–5000 LSECs. Merged phase-contrast (hepatocytes) and fluorescent (LSECs) images taken 7 days post-LSEC seeding. (C) Hepatocytes–LSECs, (D) Hepatocytes–5 PE Layers–5000 LSECs, (E) Hepatocytes–5 PE Layers–10,000 LSECs, and (F) Hepatocytes–15 PE Layers–5000 LSECs. Scale bar = 100 μm. LSEC, liver sinusoidal endothelial cell.
