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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Oncogene. 2007 Jun 4;26(54):7544–7551. doi: 10.1038/sj.onc.1210569

Figure 2.

Figure 2

Interaction of CtBP with cellular factors and E1A. (a) Interaction with cellular factors. HeLa cells were transfected with different CtBP constructs for 24 h, and cell lysates were immunoprecipitated with the Flag antibody and examined by western blots using indicated antibodies. (b) Interaction with E1A and cellular factors. A total of 293 cells were transfected with indicated CtBP plasmids, and CtBP complexes were analysed as in (a). Acetylated CtBP2 (Ac-CtBP2) was detected by the pan-Ac-Lys antibody. (c) GST pull-down assay. GST-E1A was bound to glutathione-agarose beads, and incubated with H6-tagged CtBP2, M48A or A58E mutants. CtBP2 proteins in the bound and unbound fractions were analysed by western blot using the CtBP2 antibody. GST-E1A was detected by Coomassie blue staining.