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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Oncogene. 2007 Jun 4;26(54):7544–7551. doi: 10.1038/sj.onc.1210569

Figure 3.

Figure 3

Effect of E1A-induced CtBP2 acetylation on CtBP2 binding to p300. (a) Scheme of the procedure. Flag-HA-p300 was bound to Flag antibody beads, and incubated with H6-tagged CtBP2 and 3KR-CtBP2 in the presence or absence of GST-E1A and Ac-CoA. (b) Binding of CtBP2 with p300. The fraction bound to the p300-containing beads and the supernatant fraction (20% loaded compared to the bound fraction) were examined by western blots as indicated. CtBP2 and GST-E1A were detected by western blots. Acetylated CtBP2 (Ac-CtBP2) was detected with the pan-Ac-Lys antibody. p300 was detected by silver staining. (c) Quantification of CtBP2 binding with p300. Total amount of Ac-CtBP2 was normalized to the level of Ac-CtBP2 in the presence of GST-E1A (lanes 4 and 4s).