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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Oncogene. 2007 Jun 4;26(54):7544–7551. doi: 10.1038/sj.onc.1210569

Figure 4.

Figure 4

Effect of E1A mutations on CtBP2 acetylation. (a) In vitro binding of CtBP2 and E1A mutants. In vitro binding was performed by the GST pull-down assay as described in Figure 2c. CtBP2 was detected by western blot and GST-E1A detected by Coomassie blue staining. (b) Effects of E1A on CtBP2 acetylation and association with p300. The assay was performed under conditions described in Figure 3, and various proteins were detected by western blots with the antibodies indicated in Figure 3. p300 was detected with the Flag antibody. (c) Interaction of GST-E1A with acetylated CtBP2. The binding studies were carried out under conditions described in Figure 4b, except GST-E1A proteins were first bound to glutathione beads. p300 was detected with the p300 monoclonal antibody and GST-E1A was detected by Coomassie blue staining.