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. Author manuscript; available in PMC: 2010 Aug 25.
Published in final edited form as: Oncogene. 2007 Jun 4;26(54):7544–7551. doi: 10.1038/sj.onc.1210569

Figure 6.

Figure 6

Effect of E1A on subcellular localization of CtBP1. (a) Immunofluorescence analysis. HeLa cells were transfected with different GFP-E1A plasmids along with CtBP1 or CtBP2. Transfected cells were stained with Flag-Cy3 antibody for the localization of CtBP proteins as in Figure 5. (b) Coimmunoprecipitation of CtBP1 and GFP-E1A. HeLa cells were co-transfected with CtBP1 and different GFP-E1A mutants as indicated. Cell lysates were immunoprecipitated with the Flag antibody and analysed by western blots with the Flag antibody to detect CtBP1 or GFP antibody to detect GFP-E1A. (c) Subcellular localization of CtBP1 and CtBP2 in 293 cells. All the cells were examined by indirect immunofluorescence staining with mAbs against CtBP1 or CtBP2.