Figure 5.

IRES activities of synthetic IRESs in various cell lines. The multimeric dual luciferase constructs (ICS1–23b)₅/RPh (see Fig. 3) and (ICS2–17.2)₅/RPh (see Fig. 4) were transfected into eight different cell lines. Vertical bars represent mean normalized Photinus activities with corresponding SEMs. The following cell lines were used in this experiment: rat glioma C6, human neuroblastoma SK, mouse neuroblastoma N2a, rat neuroblastoma B104, mouse fibroblast NIH 3T3 (3T3), human cervical carcinoma HeLa, normal rat kidney NRK, and mouse muscle myoblast line C₂C₁₂.