Figure 2.

Positional Analysis of Amino Acid Composition of TMDs from Different Organelles in Fungi and Vertebrates

(A and B) The position relative to the cytosolic edge of the TMDs is on the horizontal axes, and the amino acids and organelles are on the vertical axes. Amino acids are listed in order of decreasing hydrophobicity (Goldman-Engelman-Steitz [GES] scale [Engelman et al., 1986]). Normalized residue abundance is color-coded such that white represents zero and dark blue a maximum of one. The abundance of serines in the region following the lumenal end of Golgi TMDs probably reflects the fact that this part of many Golgi enzymes forms a flexible linker that tethers the catalytic domain to the membrane (Paulson and Colley, 1989). Graphical plots for individual residues can be generated at http://www.tmdsonline.org. See Table S3 and Table S4 for numerical values.