Figure 5.

Positional Analysis of Amino Acid Volume from Different Organelles in Fungi and Vertebrates

(A and B) The mean values for residue volume (Pontius et al., 1996), at each position along the TMDs from fungi and vertebrates. Error bars indicate standard error of the mean.

(C) Independent (two sample) t tests were applied at positions along the TMDs to assess the significance of differences between the mean values of amino acid volumes for Golgi and plasma membrane proteins shown in (A) and (B).

(D) The Biological scale of Hessa and coworkers was used to define cytosolic TMD edges and thus align the TMDs from different organelles at their cytosolic ends (Hessa et al., 2005). This alignment was then used for analysis of amino acid volume along the fungal Golgi and plasma membrane TMDs. Error bars indicate standard error of the mean.

See also Figure S3.