Skip to main content
. 2010 Jun 29;19(18):3491–3501. doi: 10.1093/hmg/ddq265

Figure 3.

Figure 3.

CHD7 positively regulates rRNA transcription. (A) qRT–PCR and western blot analysis of CHD7 mRNA and protein levels following the treatment of DLD1-A2 cells with non-target siRNA pools (siNT-1/2), a CHD7 siRNA pool (siCHD7-1) and a CHD7 siRNA not present in the pool (siCHD7-2). (B) qRT–PCR for the 45S pre-rRNA in cells treated with control or CHD7 siRNA (n = 2–5). (C) qRT–PCR and western blot analysis of CHD7 mRNA and protein levels in mES cells derived from Whirligig embryos. (D) qRT–PCR for the 45S pre-rRNA in mES cells (n = 4–7). (E) qRT–PCR analysis of 45S pre-rRNA levels in DLD1-A2 cells transfected with empty vector or plasmid encoding untagged human CHD7 protein (n = 2). The FLAG-tagged CHD7 expressed in DLD1-A2 cells is not detectable with the Abcam CHD7 antibody and thus, blotting with this antibody only reveals the untagged CHD7 that was transfected (see Supplementary Material, Fig. S2). Error bars represent mean + SEM for biological replicates. *P < 0.05; **P < 0.01; ***P < 0.001 by t-test.