Figure 5.

CHD7 is associated with active rDNA repeats and counteracts rDNA promoter methylation. (A) Schematic representation of active and inactive rDNA loci showing their respective epigenetic features. Open circles represent unmethylated CpG dinucleotides and methylated CpG dinucleotides are represented as filled circles. (B) ChIP-chop analysis of FLAG-CHD7, H3K4me2 and H3K9me2 binding to the human rDNA locus in DLD1-A2 cells (n = 2). (C) Analysis of rDNA promoter methylation in DLD1-A2 cells by HpaII/MspI digest and qPCR (n = 3). (D) Analysis of rDNA promoter methylation in mES cells by HpaII/MspI digest and qPCR (n = 3). Error bars represent the mean + SEM for biological replicates. *P < 0.05 by t-test.