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Pre-rRNA levels are reduced in embryonic Chd7 mutant CHARGE tissues. (A) qRT–PCR for Chd7 and pre-rRNA in whole E10.5 Chd7 gene-trap embryos (n = 2–3). (B) qRT–PCR for the 45S pre-rRNA in Chd7 mutant tissues. (C) qRT–PCR for the 45S pre-rRNA in Chd7 mutant tissues. Error bars represent the mean + SEM for biological replicates (n = 3–4). *P < 0.05, **P < 0.01 by t-test.
