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CHD7 promotes association of treacle with rDNA. (A) ChIP analysis of treacle binding to rDNA in Chd7+/+ and Chd7−/− mES cells (n = 2). Two nucleoplasmic non-target regions are included as controls for ChIP specificity. (B) qRT–PCR and western blot analysis of Tcof1 mRNA and treacle protein levels, respectively, in Chd7+/+ and Chd7−/− mES cells (n = 3). Error bars represent the mean + SEM for biological replicates. *P < 0.05 by t-test.
