Fig. 3.

The expression of Arg1 is increased in BCG-infected macrophages from Il10^−/− mice compared to that in cells from wild-type mice. (A to C) BMDMs from wild-type, Stat6^−/−, Myd88^−/−, and Il10^−/− mice were left untreated or were infected with BCG (at MOIs of 100, 10, and 1). RNA was analyzed by gel electrophoresis followed by Northern blotting (A, MOIs of 100 and 10 are shown) or by qRT-PCR (C). (B) Culture supernatants from the above BCG-infected cells were collected and analyzed by ELISA for the presence of IL-6 (data from cells infected at MOIs of 100 and 10 are shown). Data are presented as the mean concentration of IL-6 (ng/ml) ± SD. (D) Culture supernatants from wild-type and Il10^−/− BMDMs infected with BCG (combined supernatants from infections at MOIs of 100 and 10) were used to stimulate BMDMs from wild-type mice for 4 and 24 hours. Whole-cell lysates were analyzed by Western blotting for the indicated proteins. (E) Supernatants were collected from wild-type BMDMs infected with BCG (at an MOI of 10) for 24 hours. Supernatants were diluted 1:2 and used to stimulate BMDMs from wild-type;Tie2cre mice (n = 6 mice) or Socs3^flox/flox;Tie2cre mice (n = 10 mice). Twenty-four hours after stimulation, RNA was analyzed by qRT-PCR. Data are from one experiment with at least three mice per group (A to D) or from two experiments combined and presented as the mean expression ± SD (E).