Skip to main content
. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Chem. 2010 Jul 18;2(9):772–779. doi: 10.1038/nchem.733

Figure 2.

Figure 2

tRNA octamer self-assembly and thermal stability. (a) Characterization of tRNA supramolecular assembly of various tRNA octamers (TO) by non-denaturing PAGE at 2 mM Mg(OAc)2: lane M is for the monomer tRNA unit control (400 nM), lane TS1 are for a control tRNA square (TS1, 100 nM), lanes TO1–2 show octamer products (50 nM) after one pot or stepwise assembly. Lanes TO3–4 to TO3–7 (50 nM) correspond to octamers with a varying number of tail connectors. (b) Schematic of the three major transitions of TO disassembly observed by TGGE analysis (Materials and Methods and Supplementary Information). (c) Example of TGGE gel showing the cooperative biphasic dissociation of TO3–4 (20 nM) into squares and monomers at 0.2 mM Mg(OAc)2 (see also Table 1). (d) Comparison of melting curves for TS3 (grey squares) and TO3–4 (black circles) obtained from TGGE gels at 0.2 mM (empty symbols) and 15 mM (solid symbols) Mg(OAc)2.