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Purification of the ATPase from Aphanothece halophytica.
| Step | Total protein (mg) | Total activity (unit)a | Specific activity (unit mg-1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Membrane vesicles | 162.0 | 4315 | 26.6 | 1.0 | 100.0 |
| Solubilized protein | 55.5 | 3720 | 67.0 | 2.5 | 86.2 |
| PEG 6000 precipitation | 4.4 | 1062 | 241.4 | 9.1 | 24.6 |
| Superose 6 | 0.6 | 280 | 466.7 | 17.5 | 6.5 |
a One unit corresponded to 1 nmol of inorganic phosphate produced per min.
