Figure 2.

Effect of LXR activation and cholesterol synthesis inhibition on membrane and nuclear SREBPs in McA-RH7777 cells. On day 0, cells were set up and treated identically to those described in Fig. 1. On day 3, after a 16-h incubation at 37°C, cells received ALLN at 25 μg/ml and were harvested 1 h later. Aliquots of membrane and nuclear extract fractions (for A and B: Upper, 60 μg protein per lane; Lower, 80 μg protein/lane) were subjected to SDS/PAGE. Immunoblot analysis was carried out with 5 μg/ml rabbit anti-rat SREBP-1 (A) or anti-rat SREBP-2 (B). Filters were exposed for 5–10 s at room temperature. P, SREBP precursor forms; N, nuclear cleaved forms of SREBP-1c (A) and SREBP-2 (B).