Table 1.
The PCR primers used in the study
| SNP/gene | Primer sequence (5' →3') | Size (bp) | Restriction enzyme | Reference |
|---|---|---|---|---|
| MMP-3-1612 5A/6A | GATTACAGACATGGGTCACG | 120 | Xmn I | Shibata et al, 2005 |
| TTTCAATCAGGACAAGACGAAGTTT | 6A: 120 bp | |||
| 5A: 97 bp + 23 bp | ||||
| MMP-7-181 A/G | TGGTACCATAATGTCCTGAAT | 150 | EcoR I | Jormsjö et al, 2001 |
| TCGTTATTGGCAGGAAGCACACAATGAATT | A: 150 bp | |||
| G: 120 bp + 30 bp | ||||
| MMP-9exon6 A/G | CCATCCATGGGTCAAAGAAC | 295 | Sma I | Shibata et al, 2005 * |
| GGGCTGAACCTGGTAGACAG | A: 295 bp | |||
| G: 192 bp + 103 bp | ||||
| TIMP-1 372 C/T | GCACATCACTACCTGCAGTC | 175 | BssSI | Wollmer et al, 2002 |
| GAAACAAGCCCACGATTTAG | T: 175 bp | |||
| C: 152 bp + 23 bp | ||||
| TIMP-2 -418 G/C | CGTCTCTTGTTGGCTGGTCA | 304 | BsoBI | Zhou et al, 2004 |
| CCTTCAGCTCGACTCTGGAG | C: 253 bp + 51 bp | |||
| G: 230 bp + 51 bp + 23 bp | ||||
| jhp0917_1 | TGGTTTCTACTGACAGAGCGC | 307 | - | Lu et al., 2005 |
| jhp0917_2 | AACACGCTGACAGGACAATCTCCC | |||
| jhp0917_3 | GCCTAAGACCTCAAACTAGC | 296 | - | This study * |
| jhp0917_4 | CATTCTGTCAAGAGCTACC | |||
| jhp0918_1 | CCTATATCGCTAACGCGCGCTC | 276 | - | Lu et al., |
| jhp0918_2 | AAGCTGAAGCGTTTGTAACG | 2005 | ||
| jhp0918_3 | GCTAGAAAGATCAACGGAAC | 216 | - | This study * |
| jhp0918_4 | CACTTGTCTGGCTCTCAT |
*The primers were self-designed by applying Primer 3 and the restriction enzyme was selected based on the reference of Shibata et al, 2005.