Figure 3. Palmitoylation-dependent clustering of IFITM3 in the ER.
(a,b) HeLa cells grown on coverslips in 12-well plates were transfected with 1 μg pCMV-HA, pCMV-HA-IFITM3 or pCMV-HA-IFITM3-palmΔ and stained with anti-HA (red) and TOPRO-3 (blue). Insets are enlargements of the white-squared regions. Scale bars represent 10 μm. (b) Cells were also stained with antibodies against cellular markers, calreticulin and LAMP1 (green), or were co-transfected with GFP-CD9 (green).