Table 3.
Summary of vitreous testing on 25-gauge diagnostic TSV specimens
| Patient | Cytopathology | Immunohistochemistry | Flow cytometry | Gene rearrangement | Cytokine (pg/ml) | Microbiology, PCR |
|---|---|---|---|---|---|---|
| 1 | Scattered lymphocytes, monocytes | – | CD45bright, CD19dim, monoclonal κ light chain (identical to prior lymphoma) | IgH gene rearrangement detected | IL-6 <15.6, IL-10 75.5 | Gram stain, bacterial and fungal cultures negative |
| 2 | Rare, small lymphocytes | – | No evidence of T-cell lymphoma | – | – | HSV, CMV, VZV PCR negative, gram stain, bacterial, fungal cultures negative |
| 3 | Atypical lymphoid cells with irregular nuclei, lymphocytes, monocytes | – | T-cell leukaemia/lymphoma, Abnormal CD3dim population detected | TCR gamma-gene rearrangement detected | IL-6 843, IL-10 203, IL-2 18.4 | HSV, VZV PCR negative, gram stain, bacterial, fungal cultures negative |
| 4 | Lymphocytes, macrophages | Few CD68 cells (macrophages) | No evidence of T-cell lymphoma | No TCR gene rearrangement detected | IL-6 78.4, IL-10 76.0, IL-2 <15.6 | Gram stain, bacterial and fungal cultures negative |
| 5 | Isolated atypical lymphoid cells, lymphocytes, macrophages | CD20 negative, κ+ and λ+, CD68+, CD4+, CD8 cells | CD19+κ+, CD20-λ-, consistent with B-cell lymphoma | No IgH gene rearrangement detected | IL-10 <15.6, IL-6 79.0 | Gram stain, bacterial and fungal cultures negative, CMV, HSV, VZV PCR negative |
| 6 | Lymphocytosis, negative for malignant cells | – | No evidence of B-cell lymphoma | – | IL-6 <15.6, IL-10 <23.4 | EBV PCR positive, Toxo PCR negative, Gram stain, bacterial, fungal, mycobacterial culture negative |
| 7 | No evidence of lymphoma | – | Small population of B-cells | IgH gene rearrangement detected | – | PCR for CMV, VZV, HSV, EBV, Toxo, and bacterial and fungal cultures negative. |
| 8 | No evidence of lymphoma | – | 98% T-cells, CD3+, CD4+ and CD8+ T-cells, no evidence of lymphoma | TCR gene rearrangement detected | – | PCR for CMV, VZV, HSV, EBV, Toxo, and bacterial and fungal cultures negative. |
| 9 | Atypical lymphoid cells with irregular nuclei and condensed chromatin | – | No evidence of B-cell lymphoma | IgH gene rearrangement detected | IL-10 84.8 IL-6 17.7 | CMV, HSV, VZV PCR negative, Gram stain, bacterial and fungal culture negative. |
| 10 | Insufficient quantity for analysis | – | T-cell predominance, no evidence of lymphoma | κ light chain gene rearrangement detected | – | PCR for CMV, VZV, HSV, EBV, Toxo negative, bacterial and fungal cultures negative. |
| 11 | Insufficient quantity for analysis | – | T-cell predominance, no evidence of lymphoma | κ light chain gene rearrangement detected | – | PCR for CMV, VZV, HSV, EBV, Toxo negative, bacterial and fungal cultures negative. |
| 12 | Atypical lymphoid cells with irregular nuclei | – | T-cell predominance, no evidence of lymphoma | κ light chain gene rearrangement detected | – | PCR for CMV, VZV, HSV, EBV, Toxo negative, bacterial and fungal cultures negative. |
CMV, cytomegalovirus; EBV, Epstein–Barr virus; Micro, microbiology; HSV, Herpes simplex virus; IL, interleukin; Toxo, toxoplasmosis; VZV, Varicella zoster virus.