Table 2. PCR primers.
Gene | GenBank Accession No. | Semi-Quantitative PCR primer sequence | Product size (bp) |
TRAAK | AF247042 | F: 5′-TCTCAAGGGCTTCGTTTCTGCTCT -′3R: 5′-ATTGATGCAGGCTTTGAGGCACAG -′3 | 240 |
TREK-1 | NM_001017424 | F: 5′-TGGCTGTGTACTCTTTGTGGCTCT -′3R: 5′-ACTCAGTCGCCTCCTGGTTTCTTT -′3 | 349 |
TREK-2 | NM_021161 | F: 5′-TTGTTGGCCTTGCCTACTTTGCAG -′3R: 5′-ACACACACACACACACACACAACG -′3 | 756 |