Table 2. Location of true positive variants in the genomes of mutant B. anthracis compared to the reference genome.
| Strain | Parent strain | Start position | Stop position | Reference allele | Sample allele | Locus description | Coverage | % Concordance |
| HS-2-1 | ΔANR | 6,848 | 6,848 | C | T | gyrA | 5 | 100% |
| 3,364,887 | 3,364,887 | G | A | parC | 5 | 100% | ||
| 4,009,233 | 4,009,233 | G | A | Stage 0 sporulation protein A | 6 | 100% | ||
| 4,373,941 | 4,373,941 | C | A | Hypothetical protein GBAA4810 | 6 | 100% | ||
| HS-2-5 | ΔANR | 6,848 | 6,848 | C | T | gyrA | 10 | 100% |
| 2,667,930 | 2,667,930 | T | - | Hypothetical protein GBAA2880 | 14 | 100% | ||
| 3,364,887 | 3,364,887 | G | T | parC | 9 | 100% | ||
| 4,500,491 | 4,500,491 | G | A | intergenic | 9 | 100% | ||
| HS-2-5-6 | HS-2-5 | 841,481 | 843,099 | 1618 bp | Δ | tetR family transcriptional regulator | 0 (Δ)* | 100% |
| 3,588,786 | 3,588,786 | T | C | phosphodiesterase | 14 | 100% | ||
| 4,009,850 | 4,009,873 | 24 bp | Δ | Stage 0 sporulation protein A | 11 | 100% | ||
| 4,047,733 | 4,047,733 | G | T | Hypothetical protein GBAA4446 | 21 | 100% | ||
| gerH::ery ** | 34F2 chr | 4,524,685 | 4,524,685 | - | EryR | gerHA | 45 | 100% |
| 2,099,226 | 2,099,226 | T | C | HSP20 family protein | 33 | 100% | ||
| pXO1 | 172,178 | 172,178 | 20 bp | Δ | none | 66 | 95% | |
| AP50R | 34F2 chr | 895,066 | 895,066 | A | - | csaB | 14 | 100% |
| 3,894,626 | 3,894,626 | G | A | 2,3-diketo-5-methylthiopentyl-1-phosphate enolase | 8 | 100% |
*coverage for the 5′ base (841,480) flanking the deletion was 13x and the 3′ base (843,100) flanking the deletion was 4x.
**includes both FLX and Titanium runs.