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Relative levels of Jac1 and Mdj1 in mitochondria. Immunoblot band intensities of predetermined concentrations of purified His-tagged Jac1 or Mdj1 were compared with those from mitochondrial proteins isolated from wild-type yeast cells. The indicated amounts of mitochondrial proteins and purified His-tagged Jac1 (A) or Mdj1 (B) were separated by electrophoresis and subjected to immunoblot analysis using polyclonal antibodies specific for Jac1 (A) or Mdj1 (B).
