Figure 5.
Biochemical analysis of jac1Δ32 cells. Wild-type and jac1Δ32 cells were grown at 30°C and shifted to the indicated temperatures. (A) Enzyme activities and iron content of mitochondria were determined. At least triplicate samples were analyzed for at least three independent mitochondrial preparations Wild-type enzyme activity was set at 100% and iron levels at 1. Bars indicate. the average values, and standard deviations are indicated. (B) Low-temperature absorption spectra of wild-type and jac1Δ32 were measured. The absorption maxima for cytochromes are as follows: c, 547 nm; c1, 553 nm; b, 558 nm; a and a3, 600 nm.