Figure 1.

TuMV replication factories are associated with ER and co-allign with microfilaments. Nicotiana benthamiana cells expressing mCherry-tagged TuMV-induced replication factories and ER -resident GFP (A) or the actin domain of fimbrin fused to GFP (B) observed by confocal microscopy at 4 days post-agroinfiltration. Photographs are a three-dimensional rendering of 40 1-µm thick slices that overlap by 0.5-µm. Scale bar, 10 µm.