Table 3.

Statistical Summary of Methylation Index Comparisons

Comparison between	LIT1	H19
NL (Replicates) vs. BWS	P < 0.0001	P < 0.0001
	CI: 0.439–0.477	CI: 0.285–0.332
NL (Controls) vs. BWS	P < 0.0001	P < 0.0001
	CI: 0.466–0.489	CI: 0.33–0.389
NL (TD) vs. ‘new’ NL	P = 0.066	P = 0.387
	CI: 0.0009–0.028	CI: 0.026–0.066
BWS (TD) vs. ‘new’ BWS	P < 0.0001	P = 0.55
	CI: 0.004−0.01	CI: 0.057–0.11

P values (t-tests) and 95% confidence interval of the difference between selected groups (CI); see results for details. NL indicates nonaffected individual; BWS, clinical diagnosis of Beckwith-Wiedemann Syndrome; Replicates, 30(LIT1) and 32(H19) replicate pairs assessed by four laboratory technologists; Controls, 15 unrelated, unaffected samples; TD, threshold determination; ‘new,’ controls of the 75 most recent runs in the practice setting.