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. 2010 Sep;12(5):589–600. doi: 10.2353/jmoldx.2010.090227

Figure 5.

Figure 5

The CGG RP PCR product profile can reveal both the number and sequence context of AGG elements that interrupt the CGG repeat segment. Top: Schematic representation of the impact of defined AGG sequences on the CGG RP PCR product profile for a male 30 CGG allele. Interrupting AGG elements are associated with a loss of product accumulation (and associated signal intensity) for those amplicons whose corresponding CGG primer contacts a templated AGG. Bottom: Annotation of an capillary electropherogram of a male 30 CGG allele with both CGG repeats, and the two interfering AGG sequences.