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. 2010 Sep;12(5):629–635. doi: 10.2353/jmoldx.2010.090219

Figure 2.

Figure 2

Comparison with a size-based assay. A: Summary of the positive (Pos) and negative (Neg) calls obtained with serial dilutions of a mutant A-positive cell line RNA (OCI-AML3) or in vitro transcript (mut-A) in wild-type cell line RNA (HL-60). A Pos/Neg call indicates that the results were not reproducible (below or at the limit of detection of the method). B: Representative examples of capillary electrophoresis traces. The wild-type (WT)- and mutant (M)-specific peaks are indicated. The small peaks at the beginning and end of each trace correspond to molecular weight markers.