Table 3.

Comparison of Analytical Sensitivity for the Signature NPM1 Mutations Assay Alone or Combined with the Signature AML/ETO, inv(16)-ADE Primers and Probes

	Signature NPM1 (MFI)*		Signature NPM1 + Signature AML/ETO, inv(16) ADE (MFI)*
	NPM1-WT	NPM1-Mut	NPM1-WT	NPM1-Mut	Inv16-A	Inv16-D	Inv16-E	AML1/ETO
No RNA	87	87	42	81	86	64	43	57
100% OCI-AML3	13,019	12,604	9745	10,719	110	67	83	64
HL-60 + 10% OCI-AML3	11,523	11,839	9745	10,263	65	44	77	92
HL-60 + 1% OCI-AML3	14,023	9097	10,579	7623	134	78	52	37
HL-60 + 0.1% OCI-AML3	14,025	4455	9881	3408	25	73	30	36
HL-60 + 0.01% OCI-AML3	13,745	1088	10,166	785	159	81	81	74
100% HL-60	12,465	159	9236	77	2	66	24	17
HL-60 + 2E+07 copies	10,980	12,416	10,388	11,638	97	87	102	144
HL-60 + 2E+06 copies	10,245	10,718	9108	10,432	115	134	127	97
HL-60 + 2E+05 copies	11,593	8566	10,564	8151	83	87	100	57
HL-60 + 2E+04 copies	11,511	4158	10,044	3033	112	79	140	82
HL-60 + 2E+03 copies	10,588	866	10,129	664	107	122	77	97
HL-60 + 2E+02 copies	11,287	322	10,352	190	88	104	78	91

Mut, mutant; WT, wild-type.

^*Values above the cutoff are in bold.