Figure 2.

Amplification curves of duplicates of serially diluted RNA transcripts (in RNase-free water). The gene copy number for each of the six dilutions is indicated. Standard curves were plotted with threshold cycle (Ct) values against log₁₀ concentrations of RNA transcripts. A: Representative amplification profile of the 10-fold serially diluted NP RNA transcript. The PCR efficiency of 1.818 was calculated by the LC v2.0 software. B: Corresponding amplification profile of the 10-fold serially diluted HA RNA transcript, with calculated PCR efficiency of 1.772.