Skip to main content
. 2010 Sep;12(5):644–652. doi: 10.2353/jmoldx.2010.090208

Figure 2.

Figure 2

A: Thermal denaturing profiles of molecular beacons in the presence of mismatched DNA, target DNA, or no target. B: Effect of molecular beacon concentration on the fluorescence signal in MB-based real-time PCR. Concentrations of the MB were 50 nmol/L (diamond), 200 nmol/L (circle), and 500 nmol/L (triangle). C: Effect of amplicon size on the sensitivity of the real-time PCR assay. Genomic DNA was amplified with two different primer sets: (circle) 120-bp amplicon and (diamond) 300-bp amplicon. Closed and open symbols represent genomic DNA from the cell lines H1975 and PC9, respectively.