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A: PCR with a different portion of genomic DNA from H1975 with the secondary T790M mutation. A total of 200 ng of a mixture of genomic DNAs from PC9 and H1975 were used as template. The portion of genomic DNA from H1975 was as follows: 100% (triangle); 50% (asterisk); 10% (circle); 2% (+); PCR without template (diamond); and with PC9 genomic DNA (square) as negative controls. B: Standard sequencing results of PCR products from A. Arrowheads indicate the mutation site.
