Table 1.

Design of Molecular Beacons and Synthesized Template

Synthetic nucleotides*	Sequences
T790M-MB	5′-FAM-TCGGAGCTGCATGATGAGTCCGA-Dabcyl-3′
Control-MB	5′-Cy5-CCGTGAAAAAAAAAAAAAACACGG-BHQ2–3′
Target DNA	5′-ACCTCCACCGTGCAGCTCATCATGCAGCTCATGCCCTTGGCTGCCTCCT-3′
Mismatched DNA	5′-ACCTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTGGCTGCCTCCT-3′

^*T790M mutation indicates the substitution of ACG nucleotides with ATG at codon 790 in the EGFRgene as marked in bold. Underlined bases are added to create the stem domain. T790M-MB T790M mutation specific molecular beacon; control-MB, scrambled molecular beacon; target DNA, complementary templates to T790M; mismatched DNA, single mismatched to T790M.