Table 1.
Classification Results
| Sample ID | Class FF (Eberwine) | Confidence FF (Eberwine) | Class FF (Ribo SPIA) | Confidence FF (Ribo SPIA) | Class FPET (Ribo SPIA) | Confidence FPET (Ribo SPIA) |
|---|---|---|---|---|---|---|
| Sample_A1 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_A2 | ABC | 99.9% | ABC | 99.5% | ABC | 99.3% |
| Sample_A3 | ABC | 100% | ABC | 100% | ABC | 100% |
| Sample_A4 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_A5 | ABC | 99.9% | ABC | 99.8% | ABC | 95.5% |
| Sample_A6 | GCB | 7.6% | Unclassified | 19.2% | Unclassified | 17.5% |
| Sample_A8 | ABC | 100% | ABC | 100% | ABC | 98.8% |
| Sample_B1 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_B2 | ABC | 100% | ABC | 100% | ABC | 100% |
| Sample_B3 | GCB | 1.3% | Unclassified | 11.5% | Unclassified | 72.6% |
| Sample_B7 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_B8 | GCB | 0 | GCB | 0 | ABC | 93.6% |
| Sample_B9 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_C1 | GCB | 1.1% | GCB | 1.7% | GCB | 0.4% |
| Sample_C3 | ABC | 100% | ABC | 100% | ABC | 100% |
| Sample_C5 | GCB | 0.8% | GCB | 1.5% | GCB | 1.6% |
| Sample_C6 | ABC | 99.8% | ABC | 91.7% | ABC | 98.1% |
| Sample_C7 | ABC | 90.2% | ABC | 97.1% | ABC | 99.4% |
| Sample_C9 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_D1 | ABC | 99.6% | ABC | 93.6% | ABC | 98.7% |
| Sample_D2 | GCB | 0 | GCB | 0 | GCB | 0.1% |
| Sample_D3 | ABC | 94.8% | Unclassified | 60.5% | ABC | 99.6% |
| Sample_D4 | ABC | 99.9% | ABC | 99.8% | ABC | 96.0% |
| Sample_D5 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_D6 | GCB | 0.7% | GCB | 0 | Unclassified | 19.3% |
| Sample_D7 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_D9 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_E1 | ABC | 99.3% | ABC | 99.8% | ABC | 99.9% |
| Sample_E2 | ABC | 96.4% | ABC | 96.8% | ABC | 91.3% |
| Sample_E3 | ABC | 100% | ABC | 100% | ABC | 100% |
| Sample_E4 | ABC | 100% | ABC | 100% | ABC | 99.9% |
| Sample_E5 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_E6 | ABC | 99.6% | ABC | 99.1% | Unclassified | 78.2% |
| Sample_E7 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_E8 | GCB | 0 | GCB | 0 | GCB | 0.1% |
| Sample_E9 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_F1 | GCB | 0 | GCB | 0.2% | GCB | 9.2% |
| Sample_F2 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_F3 | ABC | 99.6% | ABC | 98.6% | ABC | 98.4% |
| Sample_F4 | ABC | 100% | ABC | 100% | ABC | 100% |
| Sample_F5 | GCB | 0 | GCB | 0 | GCB | 0.3% |
| Sample_F6 | ABC | 100% | ABC | 100% | ABC | 100% |
| Sample_F9 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_G1 | GCB | 0.1% | GCB | 0.2% | GCB | 0 |
| Sample_G2 | GCB | 0 | GCB | 0 | GCB | 0 |
| Sample_G3 | ABC | 99.7% | ABC | 99.6% | ABC | 98.6% |
| Sample_G4 | GCB | 1.5% | GCB | 2.3% | GCB | 3.3% |
| Sample_G5 | ABC | 100% | ABC | 100% | ABC | 100% |
Fifty-nine DLBCL fresh frozen RNA samples analyzed by the Eberwine method were classified using a previously described model.19,23 Eleven samples did not have signatures meeting the confidence needed to call ABC or GCB and were placed into the unclassified category.
The Bayesian classifier was used to classify the FF samples amplified by the Ribo-SPIA method [column: Class FFN; the confidence is reported in Confidence (FFN)].
The Bayesian classifier was used to classify the FFPET samples amplified by the Ribo-SPIA method [column: Class FFPET; the confidence is reported in Confidence (FFPET)].