Figure 3.

Fluorescence difference plots of HRMA, and sequencing results of the 129 formalin-fixed paraffin-embedded tissues, 92-bp amplicon for exon 9 (A) and 70-bp amplicon for exon 20 (B). Sequencing results of samples indicated by arrows can be found in the lower section of each of the figures or Supplemental Figure S3 at http://jmd.amjpathol.org. The sample indicated with an asterisk, in HRMA and sequencing, is the sample found to bear a high proportion for the c.3140A>G mutation, not compatible with an heterozygote.