Table 1.
PIK3CA Oligonucleotide Sequences and Locations Used in this Study
| Exon | Primers | Primer set | Amplicon position* | Amplicon size | Amplicon Tm (°C)† | Recurrent mutations‡ | Mutation position within amplicon |
|---|---|---|---|---|---|---|---|
| 9 | F:5′-GCTCAAAGCAATTTCTACACGAGA-3′ | S9 | 180, 418, 742–180, 418, 833 | 92 bp | 81.0 | c.1624G>A | 35 |
| R:5′-TCCATTTTAGCACTTACCTGTGAC-3′ | c.1633G>A | 44 | |||||
| F:5′-ATCCAGAGGGGAAAAATATG-3′ | L9 | 180, 418, 634–180, 418, 894 | 261 bp | 82.5 | c.1624G>A | 143 | |
| R:5′-ATGCTGAGATCAGCCAAAT-3′ | c.1633G>A | 152 | |||||
| 20 | F:5′-GAGGCTTTGGAGTATTTCAT-3′ | S20 | 180, 434, 739–180, 434, 808 | 70 bp | 79.5 | c.3140A>G | 41 |
| R:5′-AATCCATTTTTGTTGTCCAG-3′ | |||||||
| F:5′-TCATTTGCTCCAAACTGACCAA-3′ | L20 | 180, 434, 534–180, 434, 885 | 352 bp | 83.5 | c.3140A>G | 246 | |
| R:5′-TGGAATCCAGAGTGAGCTTTCA-3′ |
*
According to GenBank accession no. NC_000003.10.
†
The stated Tms are according to the wild-type gDNA samples.
‡
According to GenBank accession no. NM_006218.2.