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Schematic representation of the HO promoter. Swi5p-binding sites are designated A and B and are located at −1800 and −1300, respectively. DNA probes used for electrophoretic mobility-shift and DNase I footprint analyses are indicated as probe 1 (approximately −1915 to −1350), probe 2 (−1350 to −1220), and probe 3 (−1915 to −1220). Ash1p-binding sites as determined by in vitro DNase I footprint analysis are illustrated as black boxes numbered 1 through 6.
