Figure 4.
His-Ash1p binds specifically to YTGAT. A single base alteration of the consensus at −1730 from TTGAT to TTTAT was tested for effects on His-Ash1p DNA binding. The addition of His-Ash1p to wild-type (wt) and mutant (m) probes showed that His-Ash1p bound only to the probe containing the wild-type DNA sequence.
