Figure 5.

(A) In vivo repression analysis of constructs carrying wild-type and mutant versions of ASH1. Wild-type and mutant versions of ASH1 were fused to the LexA DNA-binding domain and were tested for repression activity of HO-CAN1 in an ash1Δ strain in the presence or absence of canavanine as indicated. Cultures growing in liquid selective medium were diluted and spotted on plates from highest dilution to lowest (from left to right). SD, synthetic dextrose. (B) ASH1 C502R encodes a full-length protein. An ash1Δ strain (IH3964) was transformed with plasmids carrying a vector control (MY160, lane 1), wild-type ASH1 (MY158, lane 2), or ASH1 C502R (MY159, lane 3) and assayed for Ash1p by using anti-Ash1p peptide antibodies.